Alan URBAN

PhD in Molecular and Cellular Biology

Brain imaging specialist

36 years old

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Diploma for animal surgery

Surgery Licence - Formation en Chirurgie Expérimentale Rongeurs, IUT Clermont-Ferrand - April. 2009

Animal Licence - D.U. Formation Spéciale à l'expérimentation animale Niveau 1, Université René Descartes, Paris 5 - Jan. 2009

PhD in Molecular Biology - AREMS Laboratory

Henri Poincaré University, Nancy, FRANCE
Identification, Characterization and phylogenetic studies of RNA:Ψ-synthases acting on U2 snRNA from S. cerevisiae
Supervisor: Dr. C.BRANLANT and Prof. I.MOTORINE
French Ministry of Research and Technology Fellowship
Sept. 2003 - Sept. 2008

Master of Science in Microbiology, Enzymology and Nutrition - AREMS Laboratory

Henri Poincaré University, Nancy, FRANCE
Study of a new class of RNA:Ψ-synthase from S. cerevisiae
Supervisor: Prof. I.MOTORINE
University H. Poincaré Fellowship
Sept. 2002 - Sept. 2003

Bachelor of Science in Biochemistry

Henri Poincaré University, Nancy, FRANCE
Optimization of production and purification of Rib2p in Escherichia coli using an experimental matrix design
Supervisor: Prof. I.MOTORINE
University H. Poincaré Fellowship
Sept. 1997 - Sept. 2002

 Preclinical and Clinical Imaging

• Functional ultrasound imaging of brain activity:

- Development of fUS medical device

- In vivo imaging in normal and pathological brain (Stroke, Epilepsy, Migraine,...)

- Clinical trial

• Functional optical imaging of brain activity

- Intrinsic Optical Imaging (IOI), Laser Doppler imaging (LDI)

- with Chemical probes: Development of NIR voltage sensitive dyes (VSDs)

- with Genetically encoded indicators: Voltage Sensitive fluorescent protein (VSFP), GECI (GCaMP6)

In vivo and in vitro Fluorescence microscopy (confocal, 2-Photons)

Optogenetics Actuators & Sensors

• Full design of experimental SETUP for optogenetic experiments both in vitro and in vivo

• Molecular Virology rAAV and Lentivirus (plasmid design, validation and stereotaxic injection of viruses)

In vivo and in vitro activation or silencing of neuronal network by light activated proteins (ChR2 and variants, ArchT, Halo or Mac)

Electrophysiology

• Patch-Clamp recording in rodent brain slices

• Single-Cell RT-PCR

• EEG recording in vivo

Molecular biology, virology and biochemistry

RNA work:
Cloning, in vitro transcription (cold or radiolabelled) of tRNA, rRNA and ribozymes
Gel and/or FPLC purification
Secondary structure analysis using chemical and enzymatic probes
Gel shift - Pull-down - Northern Blot - Quantitative RT-PCR
DNA work:
S.cerevisiae Yeast genetic manipulation
PCR, cloning (GATEWAY), sequencing and mutagenesis
Probes Labelling - Southern Blot - Microarray
Protein work:
Production in E.coli (batch) and Yeast P. Pastoris (fermentor)
N15,C13 labelling for NMR & Seleno-protein production for Radiocristallography
FPLC purification using AKTA Explorer/FPLC/Prime with different chromatography column
Gel electrophoresis 1/2D - Western Blot
Nanodrop Crystallogenesis (MOSQUITO)

Information Technology (IT)

• BioInformatics tools:
- DNA & RNA sequence analysis: alignment,
- Protein: Physico-chemical analysis, 2D structural prediction (PHD) and molecular visualization (pyMOL)
• OSes: Linux, MacOSX, Windows 7
• Software: Microsoft Office - Adobe CS Photoshop, Illustrator, Dreamweaver - Imaging software (ImageJ)
• Hardware & Network: Able to assemble PC/Mac/Linux systems from parts, data management, networking, database, Website CMS

Languages

English - Fairly fluent
German - 4 years of classes
French - Native speaker

• September 2012 : Lecturer of Neurobiology at Master Biomedical Engineering ESPCI-ParisTech

From 2008 to 2012 : Assistant Professor of Biology, ESPCI
Teaching Biology, Physiology and Neurobiology to First and Second Year Students.
Lectures. Tutorship.

From 2006 to 2008 : RNA - Structure and Function
Teaching Assistant. Led discussion sessions. Use of e-learning. Graded exams.

• From 2003 to 2006 : Cellular Biology and Bioinformatic
Teaching Assistant. Prepared and led discussion and review sessions for the whole class. Also work with students in small groups or one-on-one to answer questions. Graded exams.

September 2002 : 6 months Biochemistry: Introduction to structure, enzymes and metabolism
Teaching Aid. Led discussion of homework problems and looked over student's completed homework sets.

Lastest Oral communications

•  Invited speaker: Center for Neuroscience Imaging Research, IBS, Seoul, South Korea, December 1, 2015.

 Imaging brain activity in real-time using functional brain imaging in anesthetized and freely moving rodents

 •  The 26th annual Barrels Meeting, San Diego, November 7-8, 2013.

 Chronic imaging at high spatiotemporal resolution using whole brain functional sonography.

 • XXVI Symposium on Cerebral Blood Flow, Metabolism and Function - Brain 2013 - Shanghai, China, May 20-23, 2013.

 Deciphering neuronal circuitry controlling local blood flow in cerebral cortex of rodents with optogenetics and chronic fUS.

 • Sanofi-Aventis Research Center - Chilly-Mazarin, France, June 22, 2011.

 Optogenetics: Development and Application.

 • IEEE International Ultrasonics Symposium 2011, Caribe Royale, Orlando, Florida, USA, October 18-21 2011.

 Release and monitoring of composite droplets in rats liver and brain with imaging pulses.

 

Lastest Posters

 • 20th Meeting of the Neuro-Vascular French Society. November 25-27, 2015, Paris, FRANCE

 Plasticité aigüe après accident vasculaire cérébral (AVC): étude des profils d’activation par imagerie fonctionnelle cérébrale par ultrasons dans un modèle murin d’occlusion de l’artère cérébrale moyenne (ACM).

• XXVIIth Internation Symposium on Cerebral Blood Flow, Metabolism and Function (BRAIN), June 27-30, Vancouver, CANADA.

Functional Ultrasound (fUS) imaging of brain hemodynamics in a rat middle-cerebral artery occlusion (MCAo) model of selective neuronal loss (SNL) mimicking transient ischemic attack (TIA)

 • Annual Society for Neuroscience Meeting, Chicago, October 17-21, 2015.

Real-time functional ultrasound imaging of brain activity on freely moving rats during active tasks.

 • European Molecular Imaging Meeting (EMIM), Germany, March 18-20, 2015.

 Highly sensitive Voltage Sensitive Dyes emitting in Near Infrared wavelength (VSD-NIR)

 • European Pain Federation (EFIC), Austria, September 2-5, 2015.

 Deciphering maladaptative mechanisms elicited by cortical spreading depression in rats and its relationship with migraine attacks.

 • Society for Neuroscience Meeting 2013, San Diego, USA, November 9-13, 2013.

 A subclass of Fast Spiking Parvalbumin (FS-PV) interneurons expresses genes for the metallopeptidases Adamts15, Adamts8 and Neprilysin.

 • Annual Society for Neuroscience Meeting, New Orleans, USA, October 13-17, 2012.

 Chronic functional ultrasound imaging of the rat brain through a thinned skull

 Combinatorial patterns of gene expression define new classes of cortical parvalbumin interneurons in the mouse somatosensory cortex.

 • FENS meeting, Barcelona, Spain, November 14-18 2012.

 Deciphering the neuronal circuitry controlling local blood flow in the cerebral cortex of rodents with optogenetics

 • Annual Society for Neuroscience Meeting, Washington, USA, November 12-16, 2011.

 Optogenetic stimulation of PV-Interneurons triggers constriction of perforating arterioles in neocortical slices.

 • The 24th Annual Barrels Meeting, Johns Hopkins University, Baltimore, USA, November 10-11 2011.

 Optogenetic stimulation of PV-Interneurons triggers constriction of perforating arterioles in neocortical slices.

 • 10ème colloque Société des Neurosciences Française, Marseille, France, May 24-27, 2011.

 Optimisation of opsins expression in specific subclasses of cortical interneurons using recombinant Adeno-Associated Virus and Cre-driver mice.

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